Analysis of Tobacco Plant Growth (Nicotiana tabacum L.) in Growing Media Enriched with Azotobacter and Biohumic
DOI:
https://doi.org/10.31850/jgt.v15i1.1385Keywords:
tobacco plant, growing media, Azotobacter, BiohumicAbstract
The long-term dependence of tobacco farmers on synthetic chemical fertilizers may reduce soil quality and crop productivity. One of the main challenges in tobacco production is producing high-quality seedlings that can support optimal plant productivity. This study aimed to determine the best bioagent treatment to tobacco. The used a non-factorial randomized block design with four growing-media treatments: P0 (control), P1 (Azotobacter), P2 (Biohumic), and P3 (Azotobacter + Biohumic). Each treatment was replicated three times. The results showed that the combination of Azotobacter and Biohumic (P3) produced the highest mean values for all observed plant growth parameters. This response was presumably associated with the synergy between nitrogen fixation by Azotobacter and improved nutrient availability and root-zone conditions by Biohumic.
References
Ahemad, M., & Kibret, M. (2014). Mechanisms and applications of plant growth promoting rhizobacteria: Current perspective. Journal of King Saud University - Science, 26(1), 1-20.
Backer, R., Rokem, J. S., Ilangumaran, G., Lamont, J., Praslickova, D., Ricci, E., Subramanian, S., & Smith, D. L. (2018). Plant growth-promoting rhizobacteria: Context, mechanisms of action, and roadmap to commercialization of biostimulants for sustainable agriculture. Frontiers in Plant Science, 9, 1473.
Balai Penelitian Tanah. (2009). Petunjuk teknis analisis kimia tanah, tanaman, air, dan pupuk. Balai Penelitian Tanah.
Calvo, P., Nelson, L., & Kloepper, J. W. (2014). Agricultural uses of plant biostimulants. Plant and Soil, 383, 3-41.
Canellas, L. P., Olivares, F. L., Aguiar, N. O., Jones, D. L., Nebbioso, A., Mazzei, P., & Piccolo, A. (2015). Humic and fulvic acids as biostimulants in horticulture. Scientia Horticulturae, 196, 15-27.
Castelli, F., & Contillo, R. (2009). Using a chlorophyll meter to evaluate the nitrogen leaf content in flue-cured tobacco (Nicotiana tabacum L.). Italian Journal of Agronomy, 4(2), 3-11.
Elita, N., Susila, E., & Agustamar. (2022). Application of indigenous Azotobacter and Pseudomonas fluorescens bacteria to improve rice production using the SRI method. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 8, 148-153.
Fadilah, Y. F., & Usmadi. (2019). Pengaruh lama penyimpanan dan aplikasi asam humat terhadap pertumbuhan bibit tembakau (Nicotiana tabacum L.). Berkala Ilmiah Pertanian, 2(3), 127-131.
Ginting, J., & Silitonga, M. Y. (1995). Tembakau: Teknologi produksi dan pengolahan. Balai Penelitian Tembakau dan Tanaman Serat.
Hindersah, R., Yusuf, A. M., Adhiningtyas, D. I., Herdiyantoro, D., Mulyani, O., & Suryatmana, P. (2022). Azotobacter and chemical fertilizer for improving groundnut yield in field experiment. Jurnal Agrotek Tropika, 12(4), 1-10.
Lisuma, J. B., Mbega, E. R., & Ndakidemi, P. A. (2021). The effects of cultivating tobacco and supplying nitrogenous sandy soils. Agronomy Journal, 113(2), 1-10.
Marschner, P. (2012). Marschner's mineral nutrition of higher plants (3rd ed.). Academic Press.
Maryam, Rahim, I., & Suherman. (2025). Integrating biochar and fungal inputs in sandy soil systems for chili production. Integrated and Sustainable Agriculture, 1(3), 86-98.
Nardi, S., Pizzeghello, D., Muscolo, A., & Vianello, A. (2002). Physiological effects of humic substances on higher plants. Soil Biology and Biochemistry, 34(11), 1527-1536.
Nita, A., Rahim, I., Qadri, S. N., Zamzam, S., & MK, P. (2025). Karakteristik pupuk slow release berbasis biochar tongkol jagung yang diperkaya nutrisi. Journal Galung Tropika, 14(2), 212-223.
Purnomo, T., Zudri, F., Putrina, M., Fefriyanti, D. S., & Enati, N. P. (2023). Pengaruh berbagai dosis pupuk kimia dan pupuk organik cair terhadap pertumbuhan dan produksi tanaman tembakau payakumbuh (Nicotiana tabacum L.). Agrohita: Jurnal Agroteknologi Fakultas Pertanian, 8(1), 255-262.
Qadri, S. N., Yamin, M., & Darwis, D. (2023). Pertumbuhan bibit tembakau (Nicotiana tabacum L.) beberapa varietas lokal dan unggul dengan media polibag. Jurnal Galung Tropika, 12(3), 400-407.
Qadri, S. N., Yamin, M., & Darwis, D. (2024). Pengujian viabilitas dan vigor benih beberapa varietas tanaman tembakau (Nicotiana tabacum L.). Perbal: Jurnal Pertanian Berkelanjutan, 12(1), 128-136.
Rahim, I., & Ramli, Y. (2025). Identification of dominant wild aquatic plants in South Sulawesi's freshwater and their potential as biofertilizers. IOP Conference Series: Earth and Environmental Science, 1553(1), 012028.
Rahim, I., Ismirawati, N., & Rahim, A. (2025). Increasing the technology readyness level for the application of Biohumate prototype with Biochar media enriched with PGPR bacteria in dry land in Parepare City, South Sulawesi. BIO Web of Conferences, 180, 01005. https://doi.org/10.1051/bioconf/202518001005
Siswoyo, E., Masturah, R., & Fahmi, N. (2019). Bio-pestisida berbasis ekstrak tembakau dari limbah puntung rokok untuk tanaman tomat (Lycopersicum esculentum). Jurnal Presipitasi: Media Komunikasi dan Pengembangan Teknik Lingkungan, 15(2), 94-99.
Sukmawati, Bahruddin, Suherman, I., Rahim, I., Hapsa, N., Qadri, S. N., Zamzam, S., Irmayani, Munir, R. S., Kurniawan, E., Rahim, A., Ramlayani, Sukardi, Abdullah, & Sarina. (2025). Implementasi teknologi slow-release fertilizer pada usahatani tembakau Kelompok Tani Mamminasa Deceng di Kabupaten Soppeng. Jurnal Dinamika Pengabdian, 10(2), 269-278.
Syarovy, M., Purba, A., Hidayat, T. C., & Hidayat, F. (2022). Peningkatan efektivitas pupuk anorganik tunggal dengan pemberian asam humat di pembibitan tanaman karet. Prosiding Seminar Nasional Pembangunan dan Pendidikan Vokasi Pertanian, 3(1), 1-10.
Taiz, L., Zeiger, E., Moller, I. M., & Murphy, A. (2015). Plant physiology and development (6th ed.). Sinauer Associates.
Wahyuni, M., Triani, A., & Sembiring, M. (2020). Pengaruh kompos Mucuna bracteata dan Azotobacter terhadap pertumbuhan dan kadar nitrogen bibit kelapa sawit. Agrotekma: Jurnal Agroteknologi dan Ilmu Pertanian, 4(2), 1-10.
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Copyright (c) 2026 Journal Galung Tropika
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
1.png)
.png)
