Efektivitas Tepung Daun Mangrove untuk Mengendalikan Penyakit Vibriosis pada Larva Udang Vaname (Litopenaeus vannamei)
DOI:
https://doi.org/10.31850/jgt.v13i2.1243Keywords:
immersion, Rhizophora, Vibrio harveyi, immune response, THC-DHCAbstract
Shrimp production in several Asian countries, including Indonesia, accounts for more than 85% of world production, thus control of shrimp larval diseases must be strengthened to increase production. This study aimed to test the effectiveness of mangrove leaves, Rhizopora sp, in controlling larval vibriosis of the vannamei shrimp (Litopenaeus vannamei). The study began with in vitro tests to evaluate the antimicrobial activity of mangrove leaf flour against Vibrio sp bacteria at doses of 400, 500, 600, 700, 800, 900, 1000 mg/L, 5% amoxicillin antibiotic (K+) and without mangrove flour (K). In the in vivo test, vannamei shrimp larvae (mysis-3) were immersed in a solution containing mangrove leaf flour with the three best doses of in vitro test results; 800, 900, and 1000 mg/L water, plus K-, for 15 minutes according to the treatment dose and maintained for 8 days before being challenged with Vibrio spp. The parameters observed were total hemocyte count (THC), differential hemocyte count (DHC), and survival rate (SR), which were observed on days 1 and 8, respectively, with the SR parameter observed on day 7 following the challenge test. The results showed that the Mangrove leaf diet at 800 and 900 mg/L significantly improved larval resistance to the pathogen V. harveyi (P < 0.05). The high resistance was triggered by THC immunological response and the high proportion of semi-granular and hyaline at doses of 800 and 900 mg/L (P<0.05). Mangrove leaf powder can therefore suppress vibriosis disease in vanamei shrimp larvae using the immersion method at 800 and 900 mg/L concentrations.
References
Abrogueña, J.B.R., Joydas, T.V., Pappathy, M., Cali, N.A., Alcaria, J. Shoeb, M. (2020) Structure and composition of the macrobenthic community associated to shallow mangrove-seagrass habitat along the Southern Red Sea coast, Saudi Arabia. Egyptian Journal of Aquatic Research, 47(1): 61-66.
Aguirre-Guzman, G., Sanchez-Martinez, J.C., Campa-Cordova, A.I., Luna-González, A., & Ascencio, F. (2009). Penaeid shrimp immune system, The Thai Journal of Veterinary Medicine, 39, 205-215,
Anirudhan, A., Iryani, M.T.M., Andriani, Y., Sorgeloos, P., Tan, M.P., Wong, L.L., Mok, W.J., Ming, W., Yantao, L., Lau, C.C., Sung, Y.Y. (2023). The effects of Pandanus tectorius leaf extract on the resistance of White-leg shrimp Penaeus vannamei towards pathogenic Vibrio parahaemolyticus. Fish and Shellfish Immunology Reports, 4, 100101
Annisa, N., Sarjito, Prayitno, S.B. (2015). Pengaruh perendaman ekstrak daun sirih (Piper betle) dengan konsentrasi yang berbeda terhadap gejala klinis, kelulushidupan, histologi dan pertumbuhan udang vaname (Litopenaeus vannamei) yang diinfeksi Vibrio harveyi. Journal of Aquaculture Management and Technology, 4(3): 54-60
Cai, X., Jin-shun, W., Long, H., Ren, W., Zhang, X., Ai-you, H., Zhen-yu, X. (2022). The probiotic effects, dose, and duration of lactic acid bacteria on disease resistance in Litopenaeus vannamei. Aquaculture Reports, 101299
Cascarano, M.C., Stavrakidis-Zachou, O., Mladineo, I., Thompson, K.D., Papandroulakis, N., & Katharios, P. (2021). Mediterranean aquaculture in a changing climate: temperature effects on pathogens and diseases of three farmed fish species. Pathogens, 10 (9), 1205.
Chen, Y.Y., Chen, J.C., Lin, Y.C., Yeh, S.T., Huang, C.L. (2015). White shrimp Litopenaeus vannamei that have received Gracilaria tenuistipitata extract showearly recovery of immune parameters after ammonia stressing. Marine Drugs,133606–24
Dechamma, M.M., Mani, M.K., Rajeish, M., Santhosh, K.S., Akhila, D.S., Maiti, B., Bossier, P., Karunasagar, I., Karunasagar, I. (2020). Differential expression of akirin gene in black tiger shrimp Penaeus monodon in response to immunostimulant administration and infections with Vibrio harveyi and white spot syndrome virus. Journal of World Aquaculture Sociaty, 51(4):1054–1065
Fadillah, N., Waspodo, S., Azhar, F. (2019). Penambahan Ekstrak Daun Mangrove Rhizophora apiculata pada Pakan Udang Vaname (Litopenaeus vannamei) untuk Pencegahan Vibriosis. Journal of Aquaculture Science, 4 (2): 91-101
Fitri, F.A., Diantari, R., Wardiyanto. (2019). The effect of mangrove fruit (Avicennia sp.) extract on the prevention of bacterial Vibrio parahaemolyticus infection in vaname shrimp (Litopenaeus vannamei). Aquasains, (Jurnal Ilmu Perikanan dan Sumberdaya Perairan, 8(1): 735-741
Hien, T.T.T., Tao, C.T., Hoa, T.T.T., Huynh, T.G., Tu, T.L.C., Hai, T.N., Nguyen, D.H., Kim, S.H., Song, J.W., Nhan, H.T., Duc, P.M. (2022). Effects of dietary supplementation with Pro-A on growth performance, feed utilization, immune responses, and intestinal microbiota of whiteleg shrimp (Litopenaeus vannamei). Aquaculture Reports, 24, 101125.
Hoa, T.T.T., Fagnon, M.S., Duyen, T.T.M., Viet, L.Q., Chabrillat, T., Kerros, S. (2024). Dietary effect of botanical blend (Phyto Aqua Nity™) on growth, immunity and survival of Pacific White shrimps challenged against WSSV. Aquaculture Reports, 34, 101914.
Huu-The, N., Huai-Ting, H., Yu-Ru, L., Yin-Yu, C., Fan-Hua, N., Yeh-Fang, H. (2024). Dietary Galla chinensis on white shrimp Penaeus vannamei: Promotes growth, nonspecific immunity, and disease resistance against Vibrio parahaemolyticus. Aquaculture Reports, 35, 102012.
Huynh, T.G., Chiu, K.H., Chi, C.C., Cheng, C.C., Liu, C.H. (2018). A synbiotic improves the immunity of white shrimp, Litopenaeus vannamei: metabolomic analyses reveal compelling evidence. Fish and Shellfish Immunology, 79, 284–293.
Islam, M.S., Das, M., Chakroborty, K., Lee, J.M., Islam, M.R., Rafiquzzaman, S.M. (2024). Aquamimicry improves the growth performance and immunity of black tiger shrimp (Penaeus monodon) in low saline ponds. Aquaculture Reports, 36, 102082.
Kuo, H.W., Chang, C.C., Cheng, W. (2020). Innate immunity of Litopenaeus vannamei. Journal of The Fisheries Society of Taiwan, 47, 31–38.
Lin, S., Lin, X., Yang, Y., Li, F., Luo, L. (2013). Comparison of chelated zinc and zinc sulfate as zinc sources for growth and immune response of shrimp (Litopenaeus vannamei). Aquaculture, 46-47, 79-84
Madduluri, S., Rao, K.B., Sitaram, B. (2013). Invitro evaluation of antibacterial activity of five indigenous plants extractagainst five bacterial pathogens of human. International Journal of Pharmacy and Pharmaceutical Science, 5(4): 679 – 684.
Nuria, M.C., Faizatun, A. (2009). Uji Aktivitas Anti bakteri Ekstrak Etanol Daun Jarak Pagar (Jatropha curcas L) Terhadap Bakteri Staphylococcus aureus ATCC 25923, Escherichia coli ATCC ATCC 1408. Mediagro, 5(2): 26 – 37.
Poeloengan, M., Praptiwi, P. (2012). Uji aktivitas antibakteri ekstrak kulit buah manggis (Garcinia mangostana Linn). Media Litbang Kesehatan, 20 (2): 65 – 69.
Qu, K., Xiong, S., Huang, C., Jiang, X., Tian, L., Xie, S. (2024). Dietary supplementation of ornithine improved the growth, immune response and acute NH3 stress tolerance of Pacific white shrimp, Penaeus vannamei. Aquaculture Reports, 36, 102095.
Rairat, T., Chuchird, N., Keetanon, A., Carcano, P., Comi, M., Koppe, W. (2022). Effects of dietary yeast-derived nucleotide and RNA on growth performance, survival, immune responses, and resistance to Vibrio parahaemolyticus infection in Pacific white shrimp (Litopenaeus vannamei). Aquaculture Reports, 27, 101352
Saptiani, G., Prayitno, S.B., Anggarawati, S. (2021). Effect of mangrove leaf extract (Acanthus ilicifolius) on non-specific immune status and vibriosis resistance of black tiger shrimps (Penaeus monodon) challenged with Vibrio harveyi. Veterinary of World, 14(8): 2282–2289.
Shinn, A., Pratoomyot, J., Griffiths, D., Trong, T., Vu, N.T., Jiravanichpaisal, P., Briggs, M. (2018). Asian shrimp production and the economic costs of disease. Asian Fisheries Science, 31, 29–58
Yen-Chun, L., Chin-Chyuan, C., Yu-Hsuan, L., Yu-Hung, L. (2024). Effect of fermented lemon peel as a functional feed additive on growth, non-specific immune responses, and Vibrio alginolyticus resistance in whiteleg shrimp, Litopenaeus vannamei. Aquaculture Reports, 34, 101918
Zupi?i?, I.G., Orai?, D., Kri?anovi?, K., Zrn?i?, S. (2024). Whole genome sequencing of Vibrio harveyi from different sites in the Mediterranean Sea providing data on virulence and antimicrobial resistance genes. Aquaculture, 581, 740439
Downloads
Published
How to Cite
Issue
Section
Citation Check
License
Copyright (c) 2024 Journal Galung Tropika
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.