Formation of Initial Population of Wheat M0 Mutant (Triticum Aestivum L.) Using the Mutagen Colchicine

Abstract

Wheat imports are increasing due to the demand for wheat consumption, especially processed wheat, but not balanced by the production of wheat seed raw materials. To overcome this problem, it is necessary to assemble New Superior Varieties of wheat that are adaptive to Indonesian environmental conditions. This VUB assembly begins with the formation of an initial population conventionally, one of which can be through the use of chemical mutagens, namely colchicine. This study aims to obtain an effective colchicine concentration in increasing genetic variation, increasing genetic variation in the initial population of M₀ wheat mutants, obtaining morphophysiological characters that have high coefficients of diversity, and obtaining M₁ wheat seeds as candidates for the next generation. This research method begins with the selection stage of wheat parent seeds from several wheat varieties based on the physical aspects of the seeds and the characteristics of the yield components, soaking the sprouts using a colchicine mutagen solution, and planting the sprouts from the selected wheat parent seeds using a randomized block design consisting of five colchicine mutagen soaking treatments, namely without soaking; 400 ppm colchicine soaking; 600 ppm colchicine soaking; 800 ppm colchicine soaking; and 1000 ppm colchicine soaking. Each treatment was repeated three times. The results showed that 400 ppm of colchicine mutagen was an effective and dominant concentration for increasing genetic variation based on growth percentage, plant height, leaf number, and leaf length. The use of colchicine mutagens increased genetic variation due to its polyploidy properties, which are determined by the number of stomata formed.