Polimorfisme Primer RAPD pada Tanaman Jambu Mete Asal Tiga Kabupaten di Sulawesi Tenggara

Authors

  • Mirza Arsiaty Arsyad Universitas Halu Oleo
  • I Irwan Universitas Halu Oleo
  • Dirvamena Boer Universitas Halu Oleo
  • Yuni Fitri Cahyaningsih Balai Penelitian Tanaman Buah Tropika
  • Siti Halimah Larekeng Universitas Hasanuddin
  • I Iswanto Universitas Hasanuddin

DOI:

https://doi.org/10.31850/jgt.v11i2.888

Keywords:

Cashew DNA, Polymorphism, RAPD

Abstract

Southeast Sulawesi is one of the largest producers of cashew commodities in Indonesia, which has an essential meaning in the world of trade. Determination of the polymorphism of a primer is the first step in conducting genetic diversity studies to obtain genetic information about a species. This study aims to determine the polymorphic RAPD primers to be used in the analysis of the genetic diversity of cashew in three districts in Southeast Sulawesi. The study used 20 RAPD primers to amplify cashew DNA samples and obtained three RAPD primers (OPA-15, OPP-08, and OPC-11 primers) which showed polymorphic bands in the DNA samples used. The three RAPD primers obtained have the potential to be used in genetic studies of cashew originating from Southeast Sulawesi.

Author Biographies

Mirza Arsiaty Arsyad, Universitas Halu Oleo

Jurusan Agroteknologi, Fakultas Pertanian, Universitas Halu Oleo

I Irwan, Universitas Halu Oleo

Jurusan Agroteknologi, Fakultas Pertanian, Universitas Halu Oleo

Dirvamena Boer, Universitas Halu Oleo

Jurusan Agroteknologi, Fakultas Pertanian, Universitas Halu Oleo

Yuni Fitri Cahyaningsih, Balai Penelitian Tanaman Buah Tropika

Balai Penelitian Tanaman Buah Tropika, Solok, Sumatera Barat

Siti Halimah Larekeng, Universitas Hasanuddin

Fakultas Kehutanan, Universitas Hasanuddin

I Iswanto, Universitas Hasanuddin

Fakultas Kehutanan, Universitas Hasanuddin

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Published

19-08-2022

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Section

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